Members of the La Horquetta Task Force accompanied by soldiers raided the masjid shortly after members broke fast last evening.
LifeSport Coordinator Rajaee Ali, his wife and his brother Hamid Ali were among the detainees.
They were taken to the Arima Police station for questioning.
The Ali brothers were detained by officers recently.
They were held for 72 hours and released without being charged.
The Carapo Masjid has been at the centre of a controversy surrounding the LifeSport Programme.
The programme is currently being audited by the Ministry of Finance.